MCFD2 ELISA Kit (Mouse)

CAT: 0247-OKEH03666-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03666-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Multiple coagulation factor deficiency 2
Gene Aliases
1810021C21Rik; F5f8d; LM; Lman1ip; multiple coagulation factor deficiency protein 2 homolog; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; Sdnsf.
Gene ID
193813
Accession Number
NP_647456.1
Reactivity
Mouse, Mus musculus
Target
Multiple coagulation factor deficiency protein 2 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MCFD2 ELISA Kit (Mouse) (OKEH03666) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mcfd2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Mcfd2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mcfd2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
15.63 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Specificity
Natural and recombinant Mouse Multiple coagulation factor deficiency protein 2 homolog
Protein Length
145
NCBI Gene Symbol
Mcfd2
Protein Name
Q8K5B2
Gene Name URL
Mcfd2
Nucleotide Accession Number
NM_139295.3

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