MCFD2 ELISA Kit (Human)

CAT: 0247-OKEH02484-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH02484-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Multiple coagulation factor deficiency 2, ER cargo receptor complex subunit
Gene Aliases
F5F8D; F5F8D2; LMAN1IP; multiple coagulation factor deficiency protein 2; neural stem cell-derived neuronal survival protein; SDNSF.
Gene ID
90411
Accession Number
NP_001164977.1
Reactivity
Homo sapiens, Human
Target
Multiple coagulation factor deficiency protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MCFD2 ELISA Kit (Human) (OKEH02484) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MCFD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MCFD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MCFD2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
13 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Specificity
Natural and recombinant Human Multiple coagulation factor deficiency protein 2
Protein Length
146
NCBI Gene Symbol
MCFD2
Protein Name
Q8NI22
Gene Name URL
MCFD2
Nucleotide Accession Number
NM_001171506.2

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