EDAR ELISA Kit (Human)

Mean Inter-assay CV%:

7732-18-5

Ectodysplasin A receptor

Anhidrotic ectodysplasin receptor 1; DL; downless homolog; downless, mouse, homolog of; ECTD10A; ECTD10B; ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; Ectodysplasin-A receptor; ED1R; ED3; ED5; EDA1R; EDA3; EDA-A1 receptor; EDA-A1R; HRM1; tumor necrosis factor receptor superfamily member EDAR.

10913

NP_071731.1

Homo sapiens, Human

Tumor necrosis factor receptor superfamily member EDAR

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Aviva Systems Biology EDAR ELISA Kit (Human) (OKEH03542) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human EDAR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated EDAR and incubated. The EDAR found in the sample or standards competes with the biotinylated EDAR for limited binding sites on the immobilized anti-human EDAR antibody. Excess unbound biotinylated EDAR and sample or standard EDAR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated EDAR captured in the well and inversely proportional to the amount of EDAR which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

0.312-20ng/ml

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%

0.174 ng/mL

Store as indicated in product manual.

49 kDa

Natural and recombinant Human Tumor necrosis factor receptor superfamily member EDAR

448

EDAR

Q9UNE0

EDAR