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PPARG ELISA Kit (Rat)

CAT: 0247-OKEH03242-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03242-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Peroxisome proliferator-activated receptor gamma
Gene Aliases
Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; PPARgamma2.
Gene ID
25664
Accession Number
NP_001138838.1
Reactivity
Rat, Rattus norvegicus
Target
Peroxisome proliferator-activated receptor gamma
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PPARG ELISA Kit (Rat) (OKEH03242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pparg has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pparg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pparg captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
58 kDa
Specificity
Natural and recombinant Rat Peroxisome proliferator-activated receptor gamma
Protein Length
505
NCBI Gene Symbol
Pparg
Protein Name
O88275
Gene Name URL
Pparg
Nucleotide Accession Number
NM_001145366.1

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