PPARG ELISA Kit (Mouse)

CAT: 0247-OKCD02817-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02817-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma (PPARg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma (PPARg) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Peroxisome proliferator activated receptor gamma
Gene Aliases
Nr1; Nr1c3; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated receptor gamma; PPA; PPAR; Ppar-; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2.
Gene ID
19016
Accession Number
NP_001120802.1
Reactivity
Mouse|Mus musculus
Target
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed:19041764) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma (PPARg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma (PPARg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 82-94 86 EDTA Plasma (n=5) 91-98 95 Heparin Plasma (n=5) 79-99 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 82-94 86 Serum (n=5) 82-94 86 EDTA Plasma (n=5) 91-98 95 EDTA Plasma (n=5) 91-98 95 Heparin Plasma (n=5) 79-99 91 Heparin Plasma (n=5) 79-99 91
Sensitivity
0.065 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PPARG Microplate 96 Wells (12 x 8 Well strips) PPARG Lyophilized Standard 2 100X Biotinylated PPARG Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PPARG Microplate 96 Wells (12 x 8 Well strips) Anti-PPARG Microplate 96 Wells (12 x 8 Well strips) PPARG Lyophilized Standard 2 PPARG Lyophilized Standard 2 100X Biotinylated PPARG Detector Antibody 1 x 120 uL 100X Biotinylated PPARG Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg) . No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.
Protein Length
505
NCBI Gene Symbol
Pparg
Protein Name
Peroxisome proliferator-activated receptor gamma
Gene Name URL
Pparg
Nucleotide Accession Number
NM_001127330.2

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