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CRRY ELISA Kit (Mouse)

CAT: 0247-OKEH01963-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01963-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Complement component (3b/4b) receptor 1-like
Gene Aliases
Complement component receptor 1-like protein; complement receptor related protein; complement regulatory protein Crry; Cr; Crry; Mcp; mCR; mCRY; protein p65.
Gene ID
12946
Accession Number
NP_038527.2
Reactivity
Mouse, Mus musculus
Target
Complement component receptor 1-like protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CRRY ELISA Kit (Mouse) (OKEH01963) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRRY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRRY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRRY captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
53 kDa
Specificity
Natural and recombinant Mouse Complement regulatory protein Crry
Protein Length
483
NCBI Gene Symbol
Cr1l
Protein Name
Q64735
Gene Name URL
Cr1l
Nucleotide Accession Number
NM_013499.2

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