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ILVBL ELISA Kit (Human)

CAT: 0247-OKEH01772-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01772-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
IlvB acetolactate synthase like
Gene Aliases
209L8;2-hydroxyacyl-CoA lyase 1 like;2-hydroxyacyl-CoA lyase 2; acetolactate synthase homolog; acetolactate synthase-like protein; AHAS; HACL1L; ILV2H; ilvB (bacterial acetolactate synthase) -like; ilvB-like protein.
Gene ID
10994
Accession Number
NP_006835.2
Reactivity
Homo sapiens, Human
Target
Acetolactate synthase-like protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ILVBL ELISA Kit (Human) (OKEH01772) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ILVBL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ILVBL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ILVBL captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Human Acetolactate synthase-like protein
Protein Length
632
NCBI Gene Symbol
ILVBL
Protein Name
A1L0T0
Gene Name URL
ILVBL
Nucleotide Accession Number
NM_006844.4

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