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SIK2 ELISA Kit (Human)

CAT: 0247-OKEH01706-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01706-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Salt inducible kinase 2
Gene Aliases
LOH11CR1I; QIK; qin-induced kinase; Salt-inducible kinase 2; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SIK2; serine/threonine-protein kinase SNF1-like kinase 2; SIK-2; SNF1-like kinase 2; SNF1LK2.
Gene ID
23235
Accession Number
NP_056006.1
Reactivity
Homo sapiens, Human
Target
Serine/threonine-protein kinase SIK2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SIK2 ELISA Kit (Human) (OKEH01706) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SIK2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SIK2 and incubated. The SIK2 found in the sample or standards competes with the biotinylated SIK2 for limited binding sites on the immobilized anti-human SIK2 antibody. Excess unbound biotinylated SIK2 and sample or standard SIK2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SIK2 captured in the well and inversely proportional to the amount of SIK2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
103 kDa
Specificity
Natural and recombinant Human Serine/threonine-protein kinase SIK2
Protein Length
926
NCBI Gene Symbol
SIK2
Protein Name
Q9H0K1
Gene Name URL
SIK2
Nucleotide Accession Number
NM_015191.2

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