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CA1 ELISA Kit (Rat)

CAT: 0247-OKEH01284-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01284-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Carbonic anhydrase 1
Gene Aliases
Ca1; CA-I; Carbonate dehydratase I; carbonic anhydrase 1; carbonic anhydrase I.
Gene ID
310218
Accession Number
NP_001101130.1
Reactivity
Rat, Rattus norvegicus
Target
Carbonic anhydrase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CA1 ELISA Kit (Rat) (OKEH01284) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat CA1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CA1 and incubated. The CA1 found in the sample or standards competes with the biotinylated CA1 for limited binding sites on the immobilized anti-rat CA1 antibody. Excess unbound biotinylated CA1 and sample or standard CA1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CA1 captured in the well and inversely proportional to the amount of CA1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Rat Carbonic anhydrase 1
Protein Length
261
NCBI Gene Symbol
Car1
Protein Name
B0BNN3
Gene Name URL
Car1
Nucleotide Accession Number
NM_001107660.1

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