CA1 ELISA Kit (Mouse)

CAT: 0247-OKEH06872-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH06872-96WSize:96 Well
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Carbonic anhydrase 1
Gene Aliases
AW555628; Ca1; CA-I; Car; Car-1; carbonate dehydratase I; carbonic anhydrase 1; carbonic anhydrase I.
Gene ID
12346
Accession Number
NP_001077426
Reactivity
Mouse, Mus musculus
Target
Carbonic anhydrase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ca1 ELISA Kit (Mouse) (OKEH06872) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ca1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ca1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ca1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 97.7%
Sensitivity
0.421 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Carbonic anhydrase 1
NCBI Gene Symbol
Car1
Protein Name
P13634
Gene Name URL
Car1
Nucleotide Accession Number
NM_001083957

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