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ALPL ELISA Kit (Mouse)

CAT: 0247-OKEH00758-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00758-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Alkaline phosphatase, liver/bone/kidney
Gene Aliases
Ak; Akp; Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, tissue-nonspecific isozyme; ALP; APTNAP; T; TNAP; TNSALP.
Gene ID
11647
Accession Number
NP_001274101.1
Reactivity
Mouse, Mus musculus
Target
Alkaline phosphatase, tissue-nonspecific isozyme
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ALPL ELISA Kit (Mouse) (OKEH00758) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200U/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
1.61U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Mouse Alkaline phosphatase, tissue-nonspecific isozyme
Protein Length
524
NCBI Gene Symbol
Alpl
Protein Name
P09242
Gene Name URL
Alpl
Nucleotide Accession Number
NM_001287172.1

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