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ALPL ELISA Kit (Rat)

CAT: 0247-OKEH03286-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03286-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Alkaline phosphatase, biomineralization associated
Gene Aliases
Akp2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; PHOA; tissue-nonspecific alkaline phosphatase; Tissue-nonspecific ALP alkaline phosphatase; TNSALP.
Gene ID
25586
Accession Number
NP_037191.1
Reactivity
Rat, Rattus norvegicus
Target
Alkaline phosphatase, tissue-nonspecific isozyme
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology’s ALPL ELISA Kit (Human) (OKEH03286) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALPL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALPL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALPL captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200U/L
Sensitivity
1.62U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
58 kDa
Specificity
Natural and recombinant Rat Alkaline phosphatase, tissue-nonspecific isozyme
Protein Length
524
NCBI Gene Symbol
Alpl
Protein Name
P08289
Gene Name URL
Alpl
Nucleotide Accession Number
NM_013059.1

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