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CXCL10 ELISA Kit (Human)

CAT: 0247-OKEH00401-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00401-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
C-X-C motif chemokine ligand 10
Gene Aliases
10 kDa interferon gamma-induced protein; C7; crg-2; C-X-C motif chemokine 10; gamma IP10; gIP-10; IFI10; INP10; interferon-inducible cytokine IP-10; IP-10; mob-1; protein 10 from interferon (gamma) -induced cell line; SCYB10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10.
Gene ID
3627
Accession Number
NP_001556.2
Reactivity
Homo sapiens, Human
Target
C-X-C motif chemokine 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CXCL10 ELISA Kit (Human) (OKEH00401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CXCL10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CXCL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CXCL10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Human C-X-C motif chemokine 10
Protein Length
98
NCBI Gene Symbol
CXCL10
Protein Name
P02778
Gene Name URL
CXCL10
Nucleotide Accession Number
NM_001565.3

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