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CXCL10 ELISA Kit (Monkey)

CAT: 0247-OKCA00869-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00869-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
C-X-C motif chemokine 10
Gene Aliases
10 kDa interferon gamma-induced protein, C-X-C motif chemokine 10, Gamma-IP10, IP-10, SCYB10, Small-inducible cytokine B10
Gene ID
574243
Accession Number
NP_001028064.1
Reactivity
Monkey
Target
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CXCL10 ELISA Kit (Monkey) (OKCA00869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CXCL10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CXCL10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
62.5 pg/mL-4,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%)
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CXCL10
Protein Name
C-X-C motif chemokine 10
Gene Name URL
CXCL10
Nucleotide Accession Number
NM_001032892.1

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