CXCL10 ELISA Kit (Dog)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  C-X-C motif chemokine ligand 10

• Gene Aliases:

  Chemokine (C-X-C motif) ligand 10; CXC chemokine ligand 10; C-X-C motif chemokine 10; small-inducible cytokine B10.

• Gene ID:

  478432

• Accession Number:

  NP_001010949.1

• Reactivity:

  Canis lupus|Dog

• Target:

  Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3 (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma Induced Protein 10kDa (IP10) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma Induced Protein 10kDa (IP10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma Induced Protein 10kDa (IP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Gamma Induced Protein 10kDa (IP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.6-1,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 93-105 98 EDTA Plasma (n=5) 90-97 93 Heparin Plasma (n=5) 82-90 86 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-105 98 Serum (n=5) 93-105 98 EDTA Plasma (n=5) 90-97 93 EDTA Plasma (n=5) 90-97 93 Heparin Plasma (n=5) 82-90 86 Heparin Plasma (n=5) 82-90 86

• Sensitivity:

  6.0 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-CXCL10 Microplate 96 Wells (12 x 8 Well strips) CXCL10 Lyophilized Standard 2 100X Biotinylated CXCL10 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CXCL10 Microplate 96 Wells (12 x 8 Well strips) Anti-CXCL10 Microplate 96 Wells (12 x 8 Well strips) CXCL10 Lyophilized Standard 2 CXCL10 Lyophilized Standard 2 100X Biotinylated CXCL10 Detector Antibody 1 x 120 uL 100X Biotinylated CXCL10 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Notes:

  Formerly GWB-GHQYCH

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10) . No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.

• Protein Length:

  98

• NCBI Gene Symbol:

  CXCL10

• Protein Name:

  C-X-C motif chemokine 10

• Gene Name URL:

  CXCL10

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001010949.1