ELANE ELISA Kit (Human)

CAT: 0247-OKEH00275-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00275-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
CAS Number
7732-18-5
Gene Name
Elastase, neutrophil expressed
Gene Aliases
Bone marrow serine protease; ELA2; elastase 2, neutrophil; elastase-2; GE; granulocyte-derived elastase; HLE; HNE; Human leukocyte elastase; leukocyte elastase; medullasin; NE; neutrophil elastase; PMN elastase; PMN-E; polymorphonuclear elastase; polymorphonuclear leukocyte elastase; SCN1.
Gene ID
1991
Accession Number
NP_001963.1
Reactivity
Homo sapiens, Human
Target
Neutrophil elastase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ELANE ELISA Kit (Human) (OKEH00275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ELANE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ELANE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ELANE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
29 kDa
Specificity
Natural and recombinant Human Neutrophil elastase
Protein Length
267
NCBI Gene Symbol
ELANE
Protein Name
P08246
Gene Name URL
ELANE
Nucleotide Accession Number
NM_001972.2

Related Products

CatalogName
OKBB00996-96WELANE ELISA Kit (Mouse)
OKEH00276-96WELANE ELISA Kit (Mouse)
OKCD06117-96WELANE ELISA Kit (Mouse)
OKCD06118-96WELANE ELISA Kit (Rat)
OKBB00996ELANE ELISA Kit (Mouse) (OKBB00996)
OKCD06117ELANE ELISA Kit (Mouse) (OKCD06117)
OKCD06118ELANE ELISA Kit (Rat) (OKCD06118)
OKAN05649ELANE ELISA Kit (Mouse) (OKAN05649)
OKEH00276ELANE ELISA Kit (Mouse) : 96 Wells (OKEH00276)
OASA02746-200UGELANE Antibody

Alternative Products

CatalogName
E026429Human ELANE ELISA Kit
E110632Human NE; ELANE ELISA Kit
abx151392-01Human Neutrophil Elastase (ELANE) ELISA Kit
KBH20793GENLISA™ Human Neutrophil elastase (ELANE) ELISA
E0778HuHuman ELANE/Neutrophil elastase ELISA Kit
EKF57218Human ELANE (Neutrophil elastase) ELISA Kit
abx253708-01Human Neutrophil Elastase / ELA2 (ELANE) ELISA Kit
GR111339Nori® Human Chymotrypsin C/ELANE ELISA Kit
JL12352-01Human Neutrophil Elastase (NE; ELANE) ELISA Kit
MBS1752669-01Human Elastase/ELANE/ELA2 PicoKine ELISA Kit

Popular Products