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SERPINF2 ELISA Kit (Human)

CAT: 0247-OKEH00255-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00255-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Serpin family F member 2
Gene Aliases
A2AP; AAP; alpha-2-antiplasmin; alpha2AP; alpha-2-AP; ALPHA-2-PI; alpha-2-plasmin inhibitor; API; plasmin inhibitor; PLI; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2.
Gene ID
5345
Accession Number
NP_000925.2
Reactivity
Homo sapiens, Human
Target
Alpha-2-antiplasmin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINF2 ELISA Kit (Human) (OKEH00255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINF2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for SERPINF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINF2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.9375-60pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.215 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Human Alpha-2-antiplasmin
Protein Length
491
NCBI Gene Symbol
SERPINF2
Protein Name
P08697
Gene Name URL
SERPINF2
Nucleotide Accession Number
NM_000934.3

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