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CCL3 ELISA Kit (Mouse)

CAT: 0247-OKEH00163-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00163-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 3
Gene Aliases
AI323804; C-C motif chemokine 3; CCL; G0S19-; G0S19-1; heparin-binding chemotaxis protein; L2G25B; LD78; LD78alpha; macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; Mi; MIP-; MIP1; MIP1-; MIP1 (a) ; MIP1- (a) ; Mip1a; MIP1-alpha; MIP-1alpha; MIP-1-alpha; Scy; Scya3; SIS-alpha; small-inducible cytokine A3; TY-5.
Gene ID
20302
Accession Number
NP_035467
Reactivity
Mouse, Mus musculus
Target
C-C motif chemokine 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CCL3 ELISA Kit (Mouse) (OKEH00163) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
<= 4.3%
Sensitivity
7.88 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Mouse C-C motif chemokine 3
Protein Length
92
NCBI Gene Symbol
Ccl3
Protein Name
P10855
Gene Name URL
Ccl3
Nucleotide Accession Number
NM_011337

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