Ccl3 ELISA Kit (Rat)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6 6.7 5.9 6.2 8 6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6 6.7 5.9 6.2 8 6 CV (%) 6 6.7 5.9 6.2 8 6

7732-18-5

C-C motif chemokine 3

C-C motif chemokine 3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3; Ccl3; Mip1a, Scya3

25542

NP_037157.2

Rat

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

ELISA Kit

ELISA

The Aviva Quick Picokineâ„¢ Rat Ccl3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Rat Ccl3 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-A92. To measure Rat Ccl3, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The absorbance of the yellow product is linearly proportional to Rat Ccl3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl3 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Aviva's ELISA Resource Center at https://www.Avivabio.com/elisa-technical-resource-center.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernates, serum and plasma (heparin, EDTA, citrate)

7.8 pg/ml - 500 pg/ml

<1 pg/ml

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant rat Ccl3

CCL3

C-C motif chemokine 3

CCL3