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MECP2 ELISA Kit (Human)

CAT: 0247-OKDD00389-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00389-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level MECP2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level MECP2 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV_x005F Inter-Assay: CV _x005F Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Methyl-CpG binding protein 2
Gene Aliases
AUTSX3; meCp-2 protein; methyl-CpG-binding protein 2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT.
Gene ID
4204
Accession Number
NP_001104262.1
Reactivity
Homo sapiens|Human
Target
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) -containing DNA, with a preference for 5-methylcytosine (5mC) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology MECP2 ELISA Kit (Human) (OKDD00389) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MECP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MECP2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MECP2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates, other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.065 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Notes
Formerly GWB-KBBQA3
Specificity
This assay has high sensitivity and excellent specificity for detection of MECP2._x005F No significant cross-reactivity or interference between MECP2 and analogues was observed.
Protein Length
486
NCBI Gene Symbol
MECP2
Protein Name
Methyl-CpG-binding protein 2
Gene Name URL
MECP2
Nucleotide Accession Number
NM_001110792.1

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