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Mecp2 ELISA Kit (Mouse)

CAT: 0247-OKCD01685-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01685-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Methyl CpG binding protein 2
Gene Aliases
1500041B07Rik; D630021H01Rik; Mbd5; meCp-2 protein; methyl-CpG-binding protein 2; WBP1; WBP10.
Gene ID
17257
Accession Number
NP_001075448.1
Reactivity
Mouse|Mus musculus
Target
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) -containing DNA, with a preference for 5-methylcytosine (5mC) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methyl CpG Binding Protein 2 (MECP2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methyl CpG Binding Protein 2 (MECP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methyl CpG Binding Protein 2 (MECP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Methyl CpG Binding Protein 2 (MECP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.127 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Mecp2 Microplate 96 Wells (12 x 8 Well Strips) Mecp2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Mecp2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Mecp2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Mecp2 Microplate 96 Wells (12 x 8 Well Strips) Mecp2 Lyophilized Standard 2 x 20 ng Mecp2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Mecp2 Detector Antibody 120 uL 100X Biotinylated Mecp2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBWA5
Specificity
This assay has high sensitivity and excellent specificity for detection of Methyl CpG Binding Protein 2 (MECP2) . No significant cross-reactivity or interference between Methyl CpG Binding Protein 2 (MECP2) and analogues was observed.
Protein Length
484
NCBI Gene Symbol
Mecp2
Protein Name
Methyl-CpG-binding protein 2
Gene Name URL
Mecp2
Nucleotide Accession Number
NM_001081979.2

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