APLN ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Apelin (APLN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Apelin (APLN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Apelin

• Gene Aliases:

  AGTRL1 ligand; APEL; apelin; APJ endogenous ligand; XNPEP2.

• Gene ID:

  8862

• Accession Number:

  NP_059109.3

• Reactivity:

  Homo sapiens|Human

• Target:

  Endogenous ligand for the apelin receptor (APLNR) (PubMed:10525157) . Drives internalization of the apelin receptor (By similarity) . Apelin-36 dissociates more hardly than (pyroglu) apelin-13 from APLNR (By similarity) . Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity) . Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity) . Plays a role in early coronary blood vessels formation (By similarity) . Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity) . May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin (APLN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apelin (APLN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apelin (APLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apelin (APLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  125-8,000pg/mL

• Sensitivity:

  < 45pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount APLN Microplate 96 Wells (12 x 8 Well strips) APLN Lyophilized Standard 2 100X APLN HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount APLN Microplate 96 Wells (12 x 8 Well strips) APLN Microplate 96 Wells (12 x 8 Well strips) APLN Lyophilized Standard 2 APLN Lyophilized Standard 2 100X APLN HRP-Detector Anitbody 1 x 120 uL 100X APLN HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL

• NCBI Gene Symbol:

  APLN

• Protein Name:

  Apelin

• Gene Name URL:

  APLN

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_017413.4