BRCA2 ELISA Kit (Human)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  BRCA2 DNA repair associated

• Gene Aliases :

  BRCA1/BRCA2-containing complex, subunit 2; BRCC2; breast and ovarian cancer susceptibility gene, early onset; breast and ovarian cancer susceptibility protein 2; breast cancer 2 tumor suppressor; breast cancer 2, early onset; breast cancer type 2 susceptibility protein; BROVCA2; FACD; FAD; FAD1; FANCD; FANCD1; Fanconi anemia group D1 protein; GLM3; mutant BRCA2; PNCA2; truncated BRCA2 DNA repair associated; truncated BRCA2 DNA repair-associated protein; truncated breast cancer 2; truncated DNA repair-associated BRCA2; truncated DNA repair-associated protein; XRCC11.

• Gene ID :

  675

• Accession Number :

  NP_000050.2

• Reactivity :

  Homo sapiens|Human

• Target :

  Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA) . Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180) .

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Susceptibility Protein 2 (BRCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Breast Cancer Susceptibility Protein 2 (BRCA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  0.156-10ng/mL

• Sensitivity :

  < 0.060ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount BRCA2 Microplate 96 Wells (12 x 8 Well strips) BRCA2 Lyophilized Standard 2 100X BRCA2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount BRCA2 Microplate 96 Wells (12 x 8 Well strips) BRCA2 Microplate 96 Wells (12 x 8 Well strips) BRCA2 Lyophilized Standard 2 BRCA2 Lyophilized Standard 2 100X BRCA2 HRP-Detector Anitbody 1 x 120 uL 100X BRCA2 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• NCBI Gene Symbol :

  BRCA2

• Protein Name :

  Breast cancer type 2 susceptibility protein

• Gene Name URL :

  BRCA2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_000059.3