BRCA2 Antibody

• Gene Name:

  BRCA2, DNA repair associated

• Gene Aliases:

  FAD, FACD, FAD1, GLM3, BRCC2, FANCD, PNCA2, FANCD1, XRCC11, BROVCA2

• Gene ID:

  675

• Accession Number:

  NP_000050.2

• Reactivity:

  Human

• Immunogen:

  Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.

• Target:

  Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

• Clonality:

  Monoclonal

• Clone:

  5F6

• Type:

  Monoclonal Antibody

• Applications:

  IHC, IHC-F, IHC-P, WB

• Purification:

  Protein G purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid

• Buffer:

  Phosphate-buffered saline, pH 7.2, no preservative added.

• Reconstitution:

  Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

• Fragment:

  IgG1

• NCBI Gene Symbol:

  BRCA2

• Host or Source:

  Mouse

• Protein Name:

  Breast cancer type 2 susceptibility protein

• Gene Name URL:

  BRCA2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000059.3