SCT ELISA Kit (Mouse)

CAT: 0247-OKCD02872-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02872-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Secretin (SCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Secretin (SCT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Secretin
Gene Aliases
Secretin.
Gene ID
20287
Accession Number
NP_035458.1
Reactivity
Mouse|Mus musculus
Target
Stimulates formation of NaHCO (3) -rich pancreatic juice and secretion of NaHCO (3) -rich bile and inhibits HCl production by the stomach.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 101 EDTA Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 78-96 89 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 101 Serum (n=5) 96-103 101 EDTA Plasma (n=5) 96-105 101 EDTA Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 78-96 89 Heparin Plasma (n=5) 78-96 89
Sensitivity
< 4.83 pg/mL
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
133 kDa
Components
Component Amount Anti-Sct Microplate 96 Wells (12 x 8 Well strips) Sct Lyophilized Standard 2 x 1 ng 100X Sct Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-Sct Microplate 96 Wells (12 x 8 Well strips) Anti-Sct Microplate 96 Wells (12 x 8 Well strips) Sct Lyophilized Standard 2 x 1 ng Sct Lyophilized Standard 2 x 1 ng 100X Sct Biotin Complex 1 x 120 uL 100X Sct Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Secretin (SCT) . No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.
NCBI Gene Symbol
Sct
Protein Name
Secretin
Gene Name URL
Sct
Nucleotide Accession Number
NM_011328.3

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