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RUNX2 ELISA Kit (Mouse)

CAT: 0247-OKCD02856-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02856-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Runt related transcription factor 2
Gene Aliases
Acute myeloid leukemia 3 protein; AKV core binding factor; AM; AML3; Cbf; Cbfa; Cbfa1; Cbfa-1; CBF-alpha-1; core binding factor alpha 1; Core-binding factor subunit alpha-1; LS3; Oncogene AML-3; Os; Osf2; osteoblast-specific transcription factor 2; PEA2-alpha A; PEB; PEBP2 alpha A; Pebp2a1; PEBP2aA; Pebpa2a; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt-related transcription factor 2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit; transcription factor Cbfa1/Osf2.
Gene ID
12393
Accession Number
NP_001139510.1
Reactivity
Mouse|Mus musculus
Target
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1 (I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation (By similarity) . In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 2 (RUNX2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Runt Related Transcription Factor 2 (RUNX2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Runt Related Transcription Factor 2 (RUNX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Runt Related Transcription Factor 2 (RUNX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.116 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-RUNX2 Microplate 96 Wells (12 x 8 Well strips) RUNX2 Lyophilized Standard 2 100X Biotinylated RUNX2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-RUNX2 Microplate 96 Wells (12 x 8 Well strips) Anti-RUNX2 Microplate 96 Wells (12 x 8 Well strips) RUNX2 Lyophilized Standard 2 RUNX2 Lyophilized Standard 2 100X Biotinylated RUNX2 Detector Antibody 1 x 120 uL 100X Biotinylated RUNX2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 2 (RUNX2) . No significant cross-reactivity or interference between Runt Related Transcription Factor 2 (RUNX2) and analogues was observed.
Protein Length
607
NCBI Gene Symbol
Runx2
Protein Name
Runt-related transcription factor 2
Gene Name URL
Runx2
Nucleotide Accession Number
NM_001146038.2

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