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RUNX2 ELISA Kit (Rat)

CAT: 0247-OKEH05960-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05960-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Runt-related transcription factor 2
Gene Aliases
Core-binding factor subunit alpha-1; Osteoblast-specific transcription factor 2.
Gene ID
367218
Accession Number
NP_001265412.1
Reactivity
Rat, Rattus norvegicus
Target
Runt-related transcription factor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Runx2 ELISA Kit (Rat) (OKEH05960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Runx2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Runx2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Runx2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 92%
Sensitivity
0.321 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Runt-related transcription factor 2
Protein Length
218
NCBI Gene Symbol
RUNX2
Protein Name
Q9Z2J9
Gene Name URL
RUNX2
Nucleotide Accession Number
NM_001278483.1

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