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BCAR3 ELISA Kit (Human)

CAT: 0247-OKCD02398-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02398-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
BCAR3 adaptor protein, NSP family member
Gene Aliases
AND-34; BCAR3, NSP family adaptor protein; breast cancer anti-estrogen resistance 3; breast cancer antiestrogen resistance 3 protein; breast cancer anti-estrogen resistance protein 3; dJ1033H22.2 (breast cancer anti-estrogen resistance 3) ; epididymis secretory sperm binding protein; mig-7; MIG7; migration inducting gene-7; novel SH2-containing protein 2; NSP2; SH2 domain-containing protein 3B; SH2D3B.
Gene ID
8412
Accession Number
NP_001248337.1
Reactivity
Homo sapiens|Human
Target
May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 3 (BCAR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
78.12-5000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 88-105 96 EDTA Plasma (n=5) 85-92 89 Heparin Plasma (n=5) 91-104 97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-105 96 Serum (n=5) 88-105 96 EDTA Plasma (n=5) 85-92 89 EDTA Plasma (n=5) 85-92 89 Heparin Plasma (n=5) 91-104 97 Heparin Plasma (n=5) 91-104 97
Sensitivity
27 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-BCAR3 Microplate 96 Wells (12 x 8 Well strips) BCAR3 Lyophilized Standard 2 100X Biotinylated BCAR3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-BCAR3 Microplate 96 Wells (12 x 8 Well strips) Anti-BCAR3 Microplate 96 Wells (12 x 8 Well strips) BCAR3 Lyophilized Standard 2 BCAR3 Lyophilized Standard 2 100X Biotinylated BCAR3 Detector Antibody 1 x 120 uL 100X Biotinylated BCAR3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) . No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) and analogues was observed.
Protein Length
825
NCBI Gene Symbol
BCAR3
Protein Name
Breast cancer anti-estrogen resistance protein 3
Gene Name URL
BCAR3
Nucleotide Accession Number
NM_001261408.1

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