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Carnosine ELISA Kit

CAT: 0247-OKCD02258-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02258-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Carnosine (Car) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Carnosine (Car) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
L-Carnosine; Carnosine; Beta-Alanyl-L-histidine; 305-84-0; Ignotine
Reactivity
All Species
Target
L-Carnosine is a naturally occurring dipeptide neuropeptide found in muscles.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carnosine (Car) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carnosine (Car) and unlabeled Carnosine (Car) (Standards or samples) with the pre-coated antibody specific to Carnosine (Car) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carnosine (Car) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carnosine (Car) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
11.11-900ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 93-105 101 EDTA plasma (n=5) 85-92 89 heparin plasma (n=5) 88-97 92 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 93-105 101 serum (n=5) 93-105 101 EDTA plasma (n=5) 85-92 89 EDTA plasma (n=5) 85-92 89 heparin plasma (n=5) 88-97 92 heparin plasma (n=5) 88-97 92
Sensitivity
3.53 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Carnosine Microplate 96 Wells (12 x 8 Well Strips) Carnosine Lyophilized Standard 2 Carnosine Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Component Amount Component Amount Anti-Carnosine Microplate 96 Wells (12 x 8 Well Strips) Anti-Carnosine Microplate 96 Wells (12 x 8 Well Strips) Carnosine Lyophilized Standard 2 Carnosine Lyophilized Standard 2 Carnosine Biotin Complex 1 Carnosine Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Biotin Complex Reconstitution Buffer 1 x 300 uL
NCBI Gene Symbol
CAR
Protein Name
Carnosine
Gene Name URL
CAR

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