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KRT12 ELISA Kit (Human)

CAT: 0247-OKCD01517-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#: OKCD01517-96WSize: 96 Well
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AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cytokeratin 12 (CK12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cytokeratin 12 (CK12) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Keratin 12
Gene Aliases
CK-12; cytokeratin-12; K12; keratin 12, type I; keratin, type I cytoskeletal 12; Keratin-12; MECD1.
Gene ID
3859
Accession Number
NP_000214.1
Reactivity
Homo sapiens|Human
Target
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 12 (CK12) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 12 (CK12) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 12 (CK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 12 (CK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum (n=5) 78-93 81 EDTA Plasma (n=5) 90-99 95 Heparin Plasma (n=5) 78-95 85 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 78-93 81 Serum (n=5) 78-93 81 EDTA Plasma (n=5) 90-99 95 EDTA Plasma (n=5) 90-99 95 Heparin Plasma (n=5) 78-95 85 Heparin Plasma (n=5) 78-95 85
Sensitivity
< 0.058 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-KRT12 Microplate 96 Wells (12 x 8 Well Strips) KRT12 Lyophilized Standard 2 x 10 ng 100X Biotinylated KRT12 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-KRT12 Microplate 96 Wells (12 x 8 Well Strips) Anti-KRT12 Microplate 96 Wells (12 x 8 Well Strips) KRT12 Lyophilized Standard 2 x 10 ng KRT12 Lyophilized Standard 2 x 10 ng 100X Biotinylated KRT12 Detector Antibody 120 uL 100X Biotinylated KRT12 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBLN5
Specificity
This assay has high sensitivity and excellent specificity for detection of Keratin 12 (KRT12) . No significant cross-reactivity or interference between Keratin 12 (KRT12) and analogues was observed.
Protein Length
494
NCBI Gene Symbol
KRT12
Protein Name
Keratin, type I cytoskeletal 12
Gene Name URL
KRT12
Nucleotide Accession Number
NM_000223.3

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