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KRT13 ELISA Kit (Human)

CAT: 0247-OKEH07026-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07026-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Keratin 13
Gene Aliases
CK13; CK-13; cytokeratin 13; Cytokeratin-13; K13; keratin 13, type I; keratin, type I cytoskeletal 13; Keratin-13; WSN2.
Gene ID
3860
Accession Number
NP_002265
Reactivity
Homo sapiens, Human
Target
Keratin, type I cytoskeletal 13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KRT13 ELISA Kit (Human) (OKEH07026) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-KRT13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KRT13 and incubated. The KRT13 found in the sample or standards competes with the biotinylated KRT13 for limited binding sites on the immobilized anti-KRT13 antibody. Excess unbound biotinylated KRT13 and sample or standard KRT13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KRT13 captured in the well and inversely proportional to the amount of KRT13 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 98%
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Keratin, type I cytoskeletal 13
NCBI Gene Symbol
KRT13
Protein Name
P13646
Gene Name URL
KRT13
Nucleotide Accession Number
NM_002274

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