IL1B High Sensitivity ELISA Kit (Rabbit)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Interleukin 1 beta

• Gene Aliases:

  IL-1 beta; IL1-BETA; interleukin-1 beta; lymphocyte proliferation-potentiating factor.

• Gene ID:

  100008990

• Accession Number:

  NP_001075670.1

• Reactivity:

  Oryctolagus cuniculus|Rabbit

• Target:

  Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 1 Beta (IL1b) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 1 Beta (IL1b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  3.12-200pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 82-94 91 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 90-98 94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 82-94 91 Serum (n=5) 82-94 91 EDTA Plasma (n=5) 98-105 102 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 90-98 94 Heparin Plasma (n=5) 90-98 94

• Sensitivity:

  1.21 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-IL1B Microplate 96 Wells (12 x 8 Well Strips) IL1B Lyophilized Standard 2 100X Biotinylated IL1B Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-IL1B Microplate 96 Wells (12 x 8 Well Strips) Anti-IL1B Microplate 96 Wells (12 x 8 Well Strips) IL1B Lyophilized Standard 2 IL1B Lyophilized Standard 2 100X Biotinylated IL1B Detector Antibody 120 uL 100X Biotinylated IL1B Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b) . No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

• NCBI Gene Symbol:

  IL1B

• Protein Name:

  Interleukin-1 beta

• Gene Name URL:

  IL1B

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082201.1