ERBB2 ELISA Kit (Rat)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Erb-b2 receptor tyrosine kinase 2

• Gene Aliases :

  Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog) ; C-erbB-2; epidermal growth factor receptor-related protein; HER2/neu; neu; NEU proto-oncogene; p185erbB2; p185neu; proto-oncogene c-ErbB-2; proto-oncogene NEU; receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2-like; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog.

• Gene ID :

  24337

• Accession Number :

  NP_058699

• Reactivity :

  Rat|Rattus norvegicus

• Target :

  Receptor protein-tyrosine kinase

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Tyrosine Protein Kinase erbB-2 (ErbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma and other biological fluids.

• Detection Range :

  0.625-40ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) serum (n=5) 96-105 101 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 88-101 95 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 96-105 101 serum (n=5) 96-105 101 EDTA plasma (n=5) 91-99 96 EDTA plasma (n=5) 91-99 96 heparin plasma (n=5) 88-101 95 heparin plasma (n=5) 88-101 95

• Sensitivity :

  < 0.221 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Erbb2 Lyophilized Standard 2 x 40 ng 100X Biotinylated Erbb2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Erbb2 Microplate 96 Wells (12 x 8 Well Strips) Erbb2 Lyophilized Standard 2 x 40 ng Erbb2 Lyophilized Standard 2 x 40 ng 100X Biotinylated Erbb2 Detector Antibody 120 uL 100X Biotinylated Erbb2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBOO2

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor Receptor 2 (EGFR2) . No significant cross-reactivity or interference between Epidermal Growth Factor Receptor 2 (EGFR2) and analogues was observed.

• Protein Length :

  1259

• NCBI Gene Symbol :

  Erbb2

• Protein Name :

  Receptor protein-tyrosine kinase

• Gene Name URL :

  Erbb2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_017003.2