ERBB2 ELISA Kit (Human)

• Gene Name:

  Erb-b2 receptor tyrosine kinase 2

• Gene Aliases:

  CD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2.

• Gene ID:

  2064

• Accession Number:

  NP_001005862.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Receptor tyrosine-protein kinase erbB-2

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology ERBB2 ELISA Kit (Human) (OKEH02733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERBB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ERBB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERBB2 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

• Sensitivity:

  0.1 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  138 kDa

• Specificity:

  Natural and recombinant Human Receptor tyrosine-protein kinase erbB-2

• Protein Length:

  1255

• NCBI Gene Symbol:

  ERBB2

• Protein Name:

  P04626

• Gene Name URL:

  ERBB2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001005862.2