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SERPINE1 ELISA Kit (Pig)

CAT: 0247-OKCA02508-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02508-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
PLANH1
Gene ID
396945
Accession Number
NP_999075
Reactivity
Pig
Target
Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology SERPINE1 ELISA Kit (Pig) (OKCA02508) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard SERPINE1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312 - 20 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 87-97 EDTA Plasma (n=4) 98 90-102 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 87-97 Serum (n=5) 93 87-97 EDTA Plasma (n=4) 98 90-102 EDTA Plasma (n=4) 98 90-102
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SERPINE1
Protein Name
Plasminogen activator inhibitor 1
Gene Name URL
SERPINE1
Nucleotide Accession Number
NM_213910

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