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SERPINE1 ELISA Kit (Mouse)

CAT: 0247-OKEH00502-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00502-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Serine (or cysteine) peptidase inhibitor, clade E, member 1
Gene Aliases
Endothelial plasminogen activator inhibitor; Serpin E1.
Gene ID
18787
Accession Number
NP_032897.2
Reactivity
Mouse, Mus musculus
Target
Plasminogen activator inhibitor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINE1 ELISA Kit (Mouse) (OKEH00502) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse SERPINE1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse SERPINE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse SERPINE1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Mouse Plasminogen activator inhibitor 1
Protein Length
402
NCBI Gene Symbol
SERPINE1
Protein Name
P22777
Gene Name URL
SERPINE1
Nucleotide Accession Number
NM_008871.2