RNASE2 ELISA Kit (Mouse)

CAT: 0247-OKCA02447-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02447-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
MR, mR4, RNS2, Rnase2
Gene ID
53877
Accession Number
NP_001017422
Reactivity
Mouse
Target
This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Rnase2 ELISA Kit (Mouse) (OKCA02447) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rnase2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rnase2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Rnase2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
3.12 - 200 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 88 84-92 EDTA Plasma (n=4) 108 104-112 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 88 84-92 Serum (n=5) 88 84-92 EDTA Plasma (n=4) 108 104-112 EDTA Plasma (n=4) 108 104-112
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
RNASE2
Protein Name
Non-secretory ribonuclease
Gene Name URL
RNASE2
Nucleotide Accession Number
NM_001017422

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