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RNASET2 ELISA Kit (Mouse)

CAT: 0247-OKEH06461-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06461-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Ribonuclease T2A, ribonuclease T2B
Gene Aliases
0610007O07Rik;4833423A10Rik;4930532K22Rik; C79750; ribonuclease 6; ribonuclease 6-A; ribonuclease 6-B; ribonuclease T2; ribonuclease T2-A; ribonuclease T2-B; RN; Rna; RNASE6PL; Rnaset2.
Gene ID
100037283, 68195
Accession Number
NP_001008802
Reactivity
Mouse, Mus musculus
Target
Ribonuclease T2-A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Rnaset2 ELISA Kit (Mouse) (OKEH06461) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rnaset2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rnaset2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rnaset2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 93.7%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Ribonuclease T2
NCBI Gene Symbol
Rnaset2a, Rnaset2b
Protein Name
C0HKG5
Gene Name URL
Rnaset2a, Rnaset2b
Nucleotide Accession Number
NM_001008802

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