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BRAF ELISA Kit (Human)

CAT: 0247-OKCA02146-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02146-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
NS7, B-raf, BRAF1, RAFB1, B-RAF1
Gene ID
673
Accession Number
NP_004324
Reactivity
Human
Target
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology BRAF ELISA Kit (Human) (OKCA02146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BRAF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Lysates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 95 89-104 EDTA plasma (n=4) 97 90-100 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 95 89-104 Serum (n=5) 95 89-104 EDTA plasma (n=4) 97 90-100 EDTA plasma (n=4) 97 90-100
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
BRAF
Protein Name
Serine/threonine-protein kinase B-raf
Gene Name URL
BRAF
Nucleotide Accession Number
NM_004333

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