CST7 ELISA Kit (Human)

CAT: 0247-OKCA01185-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA01185-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Cystatin F
Gene Aliases
CMAP
Gene ID
8530
Accession Number
NP_003641.3
Reactivity
Human
Target
Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CST7 ELISA Kit (Human) (OKCA01185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CST7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CST7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CST7 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernates
Detection Range
0.625 - 40 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 100 94-107 EDTA Plasma (n=4) 106 98-112 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 100 94-107 Serum (n=5) 100 94-107 EDTA Plasma (n=4) 106 98-112 EDTA Plasma (n=4) 106 98-112
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CST7
Protein Name
Cystatin-F
Gene Name URL
CST7
Nucleotide Accession Number
NM_003650.3

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