CST7 ELISA Kit (Mouse)

• Gene Name:

  Cystatin F (leukocystatin)

• Gene Aliases:

  CM, Cmap

• Gene ID:

  13011

• Accession Number:

  NP_034107.2

• Reactivity:

  Mouse

• Target:

  Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Cst7 ELISA Kit (Mouse) (OKCA01650) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cst7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cst7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cst7 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  0.156 - 10 ng/mL

• Recovery:

  Sample Mean Recovery (%) Range (%) Serum (n=5) 86 83-89 EDTA Plasma (n=4) 95 91-99 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 86 83-89 Serum (n=5) 86 83-89 EDTA Plasma (n=4) 95 91-99 EDTA Plasma (n=4) 95 91-99

• Sensitivity:

  0.039 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  CST7

• Protein Name:

  Cystatin-F

• Gene Name URL:

  CST7

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009977.3