SEMA3D ELISA Kit (Mouse)

Inter-Assay CV:

7732-18-5

Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D

4631426B19Rik

108151

NP_083158.3

Mouse

Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.

ELISA Kit

Aviva Systems Biology Sema3d ELISA Kit (Mouse) (OKCA00923) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Sema3d has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sema3d is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sema3d captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates

23.5 pg/mL-1,500 pg/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 86 80-94 EDTA Plasma (n=4) 98 92-104 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 80-94 Serum (n=5) 86 80-94 EDTA Plasma (n=4) 98 92-104 EDTA Plasma (n=4) 98 92-104

5.8 pg/mL

Store as indicated in product manual.

SEMA3D

Semaphorin-3D

SEMA3D