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SEMA4D ELISA Kit (Mouse)

CAT: 0247-OKCD02880-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02880-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Gene Aliases
CD100; col; coll-4; M-sema G; Sem; Sema; sema J; Semacl2; Semaj; semaphorin H; semaphorin-4D; semaphorin-C-like 2; semaphorin-J; Semc; Semcl2.
Gene ID
20354
Accession Number
NP_001268809.1
Reactivity
Mouse|Mus musculus
Target
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) . Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 80-98 88 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 88-102 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-98 88 Serum (n=5) 80-98 88 EDTA Plasma (n=5) 98-105 102 EDTA Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 88-102 91 Heparin Plasma (n=5) 88-102 91
Sensitivity
0.30 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SEMA4D Microplate 96 Wells (12 x 8 Well strips) SEMA4D Lyophilized Standard 2 100X Biotinylated SEMA4D Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-SEMA4D Microplate 96 Wells (12 x 8 Well strips) Anti-SEMA4D Microplate 96 Wells (12 x 8 Well strips) SEMA4D Lyophilized Standard 2 SEMA4D Lyophilized Standard 2 100X Biotinylated SEMA4D Detector Antibody 1 x 120 uL 100X Biotinylated SEMA4D Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D) . No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed
Protein Length
861
NCBI Gene Symbol
Sema4d
Protein Name
Semaphorin-4D
Gene Name URL
Sema4d
Nucleotide Accession Number
NM_001281880.1

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