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PRDM16 ELISA Kit (Mouse)

CAT: 0247-OKCA00917-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00917-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
PR domain containing 16
Gene Aliases
Cs, Mel, csp1, mel1, 5730557K01Rik
Gene ID
70673
Accession Number
NP_001277955.1
Reactivity
Mouse
Target
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. May regulate granulocytes differentiation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Prdm16 ELISA Kit (Mouse) (OKCA00917) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Prdm16 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prdm16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Prdm16 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
15.6 pg/mL-1,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 86-98 EDTA Plasma (n=4) 97 90-103 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 86-98 Serum (n=5) 95 86-98 EDTA Plasma (n=4) 97 90-103 EDTA Plasma (n=4) 97 90-103
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PRDM16
Protein Name
PR domain zinc finger protein 16
Gene Name URL
PRDM16
Nucleotide Accession Number
NM_001291026.1