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CCL3 ELISA Kit (Mouse)

CAT: 0247-OKBB00722-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00722-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 74 240 18 69 249 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 CV (%) 4.1 5.4 4 4.2 7.2 5.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 74 240 18 69 249 Mean (pg/ml) 18 74 240 18 69 249 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 CV (%) 4.1 5.4 4 4.2 7.2 5.8 CV (%) 4.1 5.4 4 4.2 7.2 5.8
CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 3
Gene Aliases
C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine (C C motif) ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78-alpha (4-69), LD78ALPHA, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha (4-69), MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
Gene ID
20302
Accession Number
NP_035467.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A24-A92
Target
Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse CCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL3 has been precoated onto 96-well plates. Standards (E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CCL3 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
7.8 pg/ml - 500 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse CCL3
NCBI Gene Symbol
CCL3
Protein Name
C-C motif chemokine 3
Gene Name URL
CCL3
Nucleotide Accession Number
NM_011337.2

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