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DLL4 ELISA Kit (Rat)

CAT: 0247-OKBB01095-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01095-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 212 425 37 205 382 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 CV (%) 6.7 4.7 4.5 6.8 5.8 4.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 212 425 37 205 382 Mean (pg/ml) 34 212 425 37 205 382 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 CV (%) 6.7 4.7 4.5 6.8 5.8 4.7 CV (%) 6.7 4.7 4.5 6.8 5.8 4.7
CAS Number
7732-18-5
Gene Name
Delta-like 4 (Drosophila)
Gene Aliases
Delta 4, Delta4, Delta ligand 4, Delta like 4, DLL 4, DLL4, hdelta2, Homeobox protein DLL-4, Notch ligand delta 2, Notch ligand DLL4, XDLL-4, Q9NR61
Gene ID
311332
Accession Number
XP_006234827.1
Reactivity
Rat
Immunogen
Expression system for standard: NS0; Immunogen sequence: S28-E519
Target
Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat DLL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL4 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S28-E519) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat DLL4 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat DLL4
NCBI Gene Symbol
DLL4
Protein Name
Delta-like protein 4
Gene Name URL
DLL4
Nucleotide Accession Number
XM_006234765.2

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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