Rat DLL4 ELISA Kit PicoKine®, Carrier-free

• Background:

  Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.

• :

  heparin or EDTA

• Description:

  Rat DLL4 ELISA Kit PicoKine® (96 Tests). Quantitate Rat DLL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	34	34	35	35	34	0.5	1.4%
  Sample 2	212	221	209	203	211	6.49	3%
  Sample 3	425	416	448	431	430	11.68	2.7%

  *number of samples for each test n=16.

• Synonyms:

  Delta-like protein 4 ;Drosophila Delta homolog 4 ;Delta4 ;Dll4 ;

• Gene Name:

  Delta-like protein 4

• Gene ID:

  311332

• UniProt:

  D3ZHH1

• Reactivity:

  Rat

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S28-E519

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4 (PubMed:25700513). Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity). .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  74961 MW

• Specificity:

  Natural and recombinant rat DLL4

• Subcellular Location:

  Cell membrane ; Single-pass type I membrane protein .

• Protein Name:

  Delta-like protein 4