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CST3 ELISA Kit (Rat)

CAT: 0247-OKBB00897-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00897-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 824 2517 8104 756 2556 7350 Standard deviation 34.6 128.36 445.72 39.31 145.69 470.4 CV (%) 4.2 5.1 5.5 5.2 5.7 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 824 2517 8104 756 2556 7350 Mean (pg/ml) 824 2517 8104 756 2556 7350 Standard deviation 34.6 128.36 445.72 39.31 145.69 470.4 Standard deviation 34.6 128.36 445.72 39.31 145.69 470.4 CV (%) 4.2 5.1 5.5 5.2 5.7 6.4 CV (%) 4.2 5.1 5.5 5.2 5.7 6.4
CAS Number
7732-18-5
Gene Name
Cystatin C
Gene Aliases
ARMD11, bA218C14.4 (cystatin C), Cst3, Cystatin-3, Cystatin-C, CYTC_HUMAN, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene ID
25307
Accession Number
NP_036969.1
Reactivity
Rat
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-A140
Target
Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes) . Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin C has been precoated onto 96-well plates. Standards (NS0, M1-A140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Cystatin C amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA) and urine
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat Cystatin C
NCBI Gene Symbol
CST3
Protein Name
Cystatin-C
Gene Name URL
CST3
Nucleotide Accession Number
NM_012837.1

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