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CST3 ELISA Kit (Pig)

CAT: 0247-OKDD00620-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00620-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV_x005F Mean Inter-Assay: CV _x005F Mean Inter-Assay: CV Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Cystatin C
Gene Aliases
Cystatin-C.
Gene ID
733672
Accession Number
NP_001038067.1
Reactivity
Porcine|Sus scrofa
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology CST3 ELISA Kit (Pig) (OKDD00620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CST3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CST3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CST3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, urine and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Notes
Formerly GWB-KBBFC3
Specificity
This assay has high sensitivity and excellent specificity for detection of CST3._x005F No significant cross-reactivity or interference between CST3 and analogues was observed._x005F
Protein Length
146
NCBI Gene Symbol
CST3
Protein Name
Cystatin
Gene Name URL
CST3
Nucleotide Accession Number
NM_001044602.1

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