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TFF3 ELISA Kit (Human)

CAT: 0247-OKBB00556-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00556-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 164 425 631 152 395 640 SD 7.38 22.1 25.87 9.6 28 37.76 CV% 4.5 5.2 4.1 6.3 7.1 5.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 164 425 631 152 395 640 Mean (pg/mL) 164 425 631 152 395 640 SD 7.38 22.1 25.87 9.6 28 37.76 SD 7.38 22.1 25.87 9.6 28 37.76 CV% 4.5 5.2 4.1 6.3 7.1 5.9 CV% 4.5 5.2 4.1 6.3 7.1 5.9
CAS Number
7732-18-5
Gene Name
Trefoil factor 3 (intestinal)
Gene Aliases
HITF, hP1.B, Intestinal trefoil factor, ITF, mITF, OTTMUSP00000021729, Polypeptide P1.B, TFF3, TFF3_HUMAN, TFI, TREFOIL, Trefoil factor (intestinal), Trefoil factor 3
Gene ID
7033
Accession Number
NP_003217.3
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E22-Y80
Target
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards (E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TFF3 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human TFF3
NCBI Gene Symbol
TFF3
Protein Name
Trefoil factor 3
Gene Name URL
TFF3
Nucleotide Accession Number
NM_003226.3

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